The first step with any manuscript is to define the Topic Statement (TS), or bottom line. Next, place it in the Title, at the end of the Abstract, in the Introduction, in the Results, and twice in the Discussion. In contrast, everything but this single sentence belongs in one section (Introduction, Results, or Discussion) only. A good scientific paper must answer a hypothesis. The answer to this question is the basis for your topic statement. By repeating it throughout the manuscript, the reader is more likely to understand the significance of your work.
Ask questions to uncover your bottom line.
What was the question that you wanted to answer at the start of your project? Have you answered it? What first got you excited about your research? There was probably a big idea that intrigued you. Convey that reason and excitement in your topic statement.
Create a topic statement.
As you make the initial draft of your manuscript, think of a potential title. It should concisely answer your hypothesis. Now, think of ways to insert this title into the manuscript. In some form, it should fit at each position. In addition, each section of the manuscript should flow toward this statement. In other words, your Topic Statement structures your essay. If you need to make significant adjustments to the Topic Statement to accommodate essential parts of your essay, you may need to reword the title.